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pcrbio 1 step  (PCR Biosystems Ltd)


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    PCR Biosystems Ltd pcrbio 1 step
    Pcrbio 1 Step, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcrbio 1 step/product/PCR Biosystems Ltd
    Average 94 stars, based on 41 article reviews
    pcrbio 1 step - by Bioz Stars, 2026-03
    94/100 stars

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    (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
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    (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
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    Average 94 stars, based on 1 article reviews
    1 step - by Bioz Stars, 2026-03
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    (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

    Journal: PLOS Pathogens

    Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi

    doi: 10.1371/journal.ppat.1013849

    Figure Lengend Snippet: (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

    Article Snippet: All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures.

    Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Tandem Mass Spectroscopy